Research Interests
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ROS (Reactive Oxygen Species）
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サイトカインアッセイ
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インピーダンス計測
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細胞機能計測用デバイス
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バイオ素子
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呼吸バースト評価
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SECM(走査型電気化学顕微鏡)
Research Areas
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Nanotechnology/Materials / Green sustainable chemistry and environmental chemistry
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Life Science / Pharmaceutical hygiene and biochemistry
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Research History
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東北工業大学大学院 工学研究科電子工学専攻
2018.4
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Tohoku Institute of Technology Professor
2010.4 - 2018.3
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Tohoku Institute of Technology Associate Professor
2007
Professional Memberships
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THE ELECTROCHEMICAL SOCIETY OF JAPAN
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JAPANESE SOCIETY FOR MEDICAL AND BIOLOGICAL ENGINEERING
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JAPANESE ASSOCIATION FOR ANIMAL CELL TECHNOLOGY
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THE CHEMICAL SOCIETY OF JAPAN
Committee Memberships
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日本ポーラログラフ学会 評議員
2020.4 - 2025.3
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公益社団法人電気化学会 東北支部 幹事
2018.4
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公益社団法人電気化学会 理事
2018.4 - 2020.3
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電気化学会 代議員
2012.4 - 2017.3
Committee type：Academic society
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日本生体医工学会 ME技術教育委員
2011.4
Committee type：Academic society
Job Achievement
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入試部委員会 次長（副委員長）
2012.4 - 2016.3
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環境情報工学専攻 専攻長
2011.4 - 2017.3
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学生部委員会 次長（副委員長）
2009.4 - 2012.3
Papers
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Scanning Electrochemical Microscopy-Somatic Cell Count as a Method for Diagnosis of Bovine Mastitis Reviewed
Shigenobu Kasai, Ankush Prasad, Ryoma Kumagai, Keita Takanohashi
Biology 11 ( 4 ) 549 2022.4
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Reactive Oxygen Species Imaging in U937 Cells Reviewed
Ankush Prasad, Michaela Sedlářová, Anastasiia Balukova, Alina Ovsii, Marek Rác, Michal Křupka, Shigenobu Kasai, Pavel Pospíšil
Frontiers in Physiology 11 2020.10
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Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor Reviewed International journal
Shigenobu Kasai, Yamato Sugiura, Ankush Prasad, Kumi Y. Inoue, Teruya Sato, Tomohiro Honmo, Aditya Kumar, Pavel Pospíšil, Kosuke Ino, Yuka Hashi, Yoko Furubayashi, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata, Tomokazu Matsue
Scientific Reports 9 ( 1 ) 12234 - 12234 2019.12
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鉄ポルフィリン修飾電極を用いたデュアル型O2−•/NOセンサの構築 Reviewed
松岡 涼, 小林 ちひろ, 黒澤 覚久, 鈴木 美咲, 中山 映里, 髙橋 明美, 青柳 重夫, 相川 達男, 近藤 剛史, 葛西 重信, 湯浅 真
BUNSEKI KAGAKU 67 ( 9 ) 511 - 517 2018.9
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R. Matsuoka, C. KobayashiI, A. Nakagawa, S.Aoyagi, T. Aikawa, T. Kondo, S.Kasai, M.Yuasa
Analytical Sciences 33 ( 8 ) 911 - 915 2017.8
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Ankush Prasad, Aditya Kumar, Ryo Matsuoka, Akemi Takahashi, Ryo Fujii, Yamato Sugiura, Hiroyuki Kikuchi, Shigeo Aoyagi, Tatsuo Aikawa, Takeshi Kondo, Makoto Yuasa, Pavel Pospíšil, Shigenobu Kasai
PeerJ 5 e3050 - e3050 2017.7
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Scanning Electrochemical Microscopy Imaging during Respiratory Burst in Human Cell Reviewed
Hiroyuki Kikuchi, Ankush Prasad, Ryo Matsuoka, Shigeo Aoyagi, Tomokazu Matsue, Shigenobu Kasai
FRONTIERS IN PHYSIOLOGY 7 25 2016.2
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バイオLSIを用いた光合成活性イメージング
杉浦 大和, 橋 由佳, 井上 久美, 伊野 浩介, 末永 智一, 葛西 重信
電気関係学会東北支部連合大会講演論文集 2016 38 - 38 2016
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Simultaneous Real-Time Monitoring of Oxygen Consumption and Hydrogen Peroxide Production in Cells Using Our Newly Developed Chip-Type Biosensor Device Reviewed
Ankush Prasad, Hiroyuki Kikuchi, Kumi Y.Inoue, Makoto Suzuki, Yamato Sugiura, Tomoya Sugai, Amano Tomonori, Mika Tada, Masaki Kobayashi, Tomokazu Matsue, Shigenobu Kasai
Frontiers in Physiology 7 ( 25 ) 2016
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Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor Reviewed
Ankush Prasad, Aditya Kumar, Makoto Suzuki, Hiroyuki Kikuchi, Tomoya Sugai, Masaki Kobayashi, Pavel Pospisil, Mika Tada, Shigenobu Kasai
FRONTIERS IN PLANT SCIENCE 6 862 2015.10
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Generation mechanism of radical species by tyrosine-tyrosinase reaction Reviewed
Mika Tada, Masahiro Kohno, Shigenobu Kasai, Yoshimi Niwano
Journal of Clinical Biochemistry and Nutrition 47 ( 2 ) 162 - 166 2010.9
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Electrochemical monitoring of hydrogen peroxide released from leucocytes on horseradish peroxidase redox polymer coated electrode chip Reviewed
Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku, Shigenobu Kasai, Tomoyuki Yasukawa, Fumio Mizutani, Tomokazu Matsue
Biosensors and Bioelectronics 25 ( 7 ) 1723 - 1728 2010.3
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Maternal vitamin B12deficiency affects spermatogenesis at the embryonic and immature stages in rats Reviewed
Toshiaki Watanabe, Shuhei Ebara, Sachiko Kimura, Kunihiko Maeda, Yoshitaka Watanabe, Hiroshi Watanabe, Shigenobu Kasai, Yoshihisa Nakano
Congenital Anomalies 47 ( 1 ) 9 - 15 2007.3
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Yu Suke Torisawa, Noriko Ohara, Kuniaki Nagamine, Shigenobu Kasai, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
Analytical Chemistry 78 ( 22 ) 7625 - 7631 2006.11
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Cytokine assay on a cellular chip by combining collagen gel embedded culture with scanning electrochemical microscopy Reviewed
S Kasai, H Shiku, YS Torisawa, K Nagamine, T Yasukawa, T Watanabe, T Matsue
ANALYTICA CHIMICA ACTA 566 ( 1 ) 55 - 59 2006.4
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Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1) Reviewed
S Kasai, H Shiku, YS Torisawa, H Noda, J Yoshitake, T Shiraishi, T Yasukawa, T Watanabe, T Matsue, T Yoshimura
ANALYTICA CHIMICA ACTA 549 ( 1-2 ) 14 - 19 2005.9
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NAD(P)H oxidase plays a crucial role in PDGF-induced proliferation of hepatic stellate cells Reviewed
Tohru Adachi, Hitoshi Togashi, Akihiko Suzuki, Shigenobu Kasai, Junitsu Ito, Kazuhiko Sugahara, Sumio Kawata
Hepatology 41 ( 6 ) 1272 - 1281 2005.6
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Respiration activity of single bovine embryos entrapped in a cone-shaped microwell monitored by scanning electrochemical microscopy Reviewed
Hitoshi Shiku, Takuo Shiraishi, Shigeo Aoyagi, Yousuke Utsumi, Masahki Matsudaira, Hiroyuki Abe, Hiroyoshi Hoshi, Shigenobu Kasai, Hiroaki Ohya, Tomokazu Matsue
Analytica Chimica Acta 522 ( 1 ) 51 - 58 2004.9
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Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians Reviewed
Yusuke Torisawa, Hioshi Shiku, Shigenobu Kasai, Matsuhiko Nishizawa, Tomokazu Matsue
International Journal of Cancer 109 302 - 308 2004.1
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Huafang Zhou, Shigenobu Kasai, Hiroyuki Noda, Hiroaki Ohya-Nishiguchi, Hitoshi Shiku, Tomokazu Matsue
Bulletin of the Chemical Society of Japan 76 ( 9 ) 1757 - 1762 2003.9
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Simultaneous detection of uric acid and glucose on a dual-enzyme chip using scanning electrochemical microscopy/scanning chemiluminescence microscopy Reviewed
S Kasai, Y Hirano, N Motochi, H Shiku, M Nishizawa, T Matsue
ANALYTICA CHIMICA ACTA 458 ( 2 ) 263 - 270 2002.5
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Mapping peroxidase in plant tissues by scanning electrochemical microscopy Reviewed
Huafang Zhou, Hitoshi Shiku, Shigenobu Kasai, Hiroyuki Noda, Tomokazu Matsue, Hiroaki Ohya-Nishiguchi, Hitoshi Kamada
Bioelectrochemistry 54 151 - 156 2001.8
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Imaging localized horseradish peroxidase on a glass surface with scanning electrochemical/chemiluminescence microscopy Reviewed
HF Zhou, S Kasai, T Matsue
ANALYTICAL BIOCHEMISTRY 290 ( 1 ) 83 - 88 2001.3
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Immunoassay of the MRSA-related toxic protein, leukocidin, with scanning electrochemical microscopy Reviewed
S Kasai, A Yokota, HF Zhou, M Nishizawa, K Niwa, T Onouchi, T Matsue
ANALYTICAL CHEMISTRY 72 ( 23 ) 5761 - 5765 2000.12
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Chemiluminescence Imaging of Localized Enzymes by Scanning Chemiluminescence Microscopy Reviewed
Shigenobu Kasai, Huafang Zhou, Tomokazu Matsue
Chemistry Letters 29 ( 3 ) 200 - 201 2000.3
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Imaging the Activity of Immobilized Horse Radish Peroxidase with Scanning Electrochemical/chemiluminescence Microscopy Reviewed
H. Zhou, S. Kasai, T. Yasukawa, T. Matsue
Electrochemistry 67 ( 12 ) 1999.6
MISC
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最新のトピックス Biochemistry 呼吸バースト現象を画像化する ー細胞が産生する過酸化水素を電気化学チップで計測ー
月刊 化学 3月号 株式会社化学同人 71 ( 3 ) 68 - 69 2016.3
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チップ型電気化学バイオセンサを用いたTHP‐1細胞の貪食による呼吸バースト評価
鈴木誠, 多田美香, 小林正樹, 井上久美, 末永智一, 葛西重信
電気化学秋季大会講演要旨集 2014 203 2014.9
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低周波電気化学インピーダンス計測法を用いた免疫細胞の薬剤刺激による挙動評価
鈴木礼紀, 石井隆介, 多田美香, 丸尾容子, 加藤善大, 小林正樹, 葛西重信
電気化学秋季大会講演要旨集 2014 213 2014.9
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走査型電気化学顕微鏡を用いたTHP‐1細胞の呼吸バーストイメージング
菊地紘幸, 鈴木誠, 佐藤大輔, 本望知浩, 多田美香, 小林正樹, 井上久美, 末永智一, 葛西重信
電気化学会大会講演要旨集 81st 259 2014.3
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チップ型電気化学バイオセンサを用いたTHP‐1細胞の呼吸バースト及び過酸化水素産生の同時計測評価
鈴木誠, 菊地紘幸, 菅井智也, 本望知浩, 多田美香, 小林正樹, 井上久美, 末永智一, 葛西重信
電気化学会大会講演要旨集 81st 260 2014.3
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低周波電気化学インピーダンス計測法を用いた免疫細胞の増殖曲線評価
鈴木礼紀, 石井隆介, 多田美香, 丸尾容子, 加藤善大, 小林正樹, 葛西重信
電気化学会大会講演要旨集 81st 259 2014.3
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電気化学的手法を用いた浮遊性免疫細胞の呼吸活性および過酸化水素産生の評価
鈴木誠, 井上久美, 菊地紘幸, 中野将識, 本望知浩, 多田美香, 小林正樹, 末永智一, 葛西重信
Rev Polarogr 59 ( 3 ) 213 2013.10
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走査型電気化学顕微鏡を用いたTHP‐1細胞の呼吸バーストの定量的評価
菊地紘幸, 井上久美, 鈴木誠, 中野将識, 本望知浩, 多田美香, 小林正樹, 末永智一, 葛西重信
Rev Polarogr 59 ( 3 ) 212 2013.10
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鈴木礼紀, 石井隆介, 多田美香, 小林正樹, 葛西重信
Rev Polarogr 59 ( 3 ) 205 2013.10
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石井隆介, 鈴木礼紀, 多田美香, 小林正樹, 葛西重信
Rev Polarogr 59 ( 3 ) 214 2013.10
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多田美香, 庭野吉己, 小林正樹, 葛西重信, 伊藤智博, 尾形健明, 河野雅弘
バイオメディカル分析科学シンポジウム講演要旨集 26th 120 - 121 2013.8
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多田美香, 庭野吉己, 葛西重信, 小林正樹, 伊藤智博, 尾形健明, 河野雅弘
電子スピンサイエンス学会年会講演要旨集 51st 264 - 265 2012.11
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Fabrication and electrochemical characterization of a microcfluidic device with a separated reference electrode
長峯邦明, 沼井大輔, 安川智之, 葛西重信, 珠玖仁, 小出昌弘, 板山朋聡, 末永智一
日本化学会講演予稿集 86th ( 1 ) 2006
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Microspots of GOD-HRP Bienzyme for Scanning Chemiluminescence Microscopy with Higher Resolution
HIRANO Yu, KASAI Shigenobu, NISHIZAWA Matsuhiko, MATSUE Tomokazu
Electrochemistry 69 ( 12 ) 946 - 948 2001.12
Research Projects
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The environmental pollution material which assumed NO produced by a cultured cell an index and a food composition
Grant number：17550146 2005 - 2007
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
YOSHITAKE Jun, SHIGENOBU Kasai, TOMOKAZU Matsuei
Grant amount：\3810000 （ Direct Cost: \3600000 、 Indirect Cost：\210000 ）
Since the endocrine and immune systems share portions of some intracellular signal pathways, endocrine disrupting chemicals (EDCs) are considered potential agents for influencing inflammatory responses. Here, we investigated the effect of EDCs on lipopolysaccharide (LPS)-induced NO production and NF-κB activation in the RAW264.7 mouse macrophage cell line. Five phenol-containing EDCs were investigated, namely bisphenol A (BPA), the alkyl phenols p-n-nonylphenol (NP) and p-n-octylphenol (OP), and the chlorinated phenols 2,4-dichrolophenol (DCP) and pentachlorophenol (PCP). Our results revealed that these chemicals dose-dependently suppressed LPS-induced NO production, as reflected by decreased NOx content. The suppressive effects of BPA, NP and OP, but not PCP or DCP, were blocked by the estrogen receptor (ER) inhibitor, ICI182780. ELISA-based quantification of the DNA-binding activity of free p65 NF-κB showed that LPS-induced NF-κB activation was significantly diminished by EDC treatment. Furthermore, immunocytochemical analysis of 8-nitroguanosine, a unique index of NO-mediated signaling, showed that 8-nitroguanosine formation increased in LPS-stimulated cells, but this increase was inhibited by the tested EDCs. These results demonstrate that EDCs suppress NO production and NF-κB activation in LPS-stimulated macrophages through ER-dependent (BPA, NP, OP) and -independent (PCP, DCP) pathways. The EDCs further inhibited 8-nitroguanosine formation, suggesting that they interfere with NO-mediated signaling. Thus, EDCs might play important roles in the inflammatory response and host defense system against foreign pathogens.
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Physiological action of nitric oxide produced by a stimulation of endotoxin from Helicobacter pylori on gastric mucosa
Grant number：15590087 2003 - 2004
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
YOSHIMURA Tetsuhiko, KASAI Shigenobu, MORITA Hidetoshi
Grant amount：\3600000 （ Direct Cost: \3600000 ）
Helicobacter pylori, a gram-negative bacterium, is the primary cause of gastritis and a major contributor to peptic ulcer disease. Despite its importance as a human pathogen, principal factor of pathogenicity of the bacterium remains to be identified Nitric oxide (NO) released due to the induction of Ca^<2+> independent isoform of nitric oxide synthase (iNOS) by lipopolysaocharide or endotoxin from a gram-negative bacterium bas been known to be deleterious to a gastric mucosa. In the present shady, to elucidate the physiological action of H.pylori LPS on gastric mucosa and the role of NO, we were carried out both in vivo and in vitro study by employing E.coli LPS as a control.
1. IT pylori LPS was isolated from gram-order bacteria by the phenol-water extraction method, and then further purified by tine enzymatic digestion of RNA, DNA, and proteins.
2. In vitro cross talk between products from iNOS and cyclooxygenase (COX) in the rat gastric mucosa during endotoxemia was examined in vivo. The results demonstrate that in the E cob LPS-treated rat gastric mucosa, PGE2, a COX product, enhances after activation of iNOS. The effect of COX activity on iNOS-NO pathway is important in the regulation of gastric mucosal integrity inflammatory states.
3. We further elucidated the interaction of LPS with gastric epithelial cells by using a normal mouse gastric surface mucous cell line (GSM06). Our findings suggest that both LPSs from H.pylori and E.coli induce the iNOS and Toll-like receptor (TLR) 2 through the TLR 4 expressed constitutively in GSM06. This mechanism on TLR 2 induction can be crucial for maintenance of gastric mucosal integrity. -
EVELOPMENT OF MICROASSEY SYSTEM TO AIM AT ENVIRONMENTAL MONITORING.
Grant number：14550775 2002 - 2003
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
KASAI Shigenobu, MATSUE Tomokazu, TAKANO Hirohisa, NODA Hiroyuki
Grant amount：\2700000 （ Direct Cost: \2700000 ）
1).Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1)
Human monocytic leukemia cell line, THP-1, is differentiated into macrophages by phorbol myristate acetate (PMA) treatment. We report real-time monitoring of reactive oxygen species (ROSs) and cytokines production during the differentiation process. The production of ROSs by THP-1 with several hours time scale has been detected using chemiluminescence's methods.
2)Characterization of the Peroxidase Activity and hydrogen peroxide production of cell by SECM (scanning electrochemistry microscope)
The peroxidase activity in a single protoplast of alga Bryopsis plumosa is quantitatively characterized by scanning electrochemical microscopy. The generation of ferriceniummethanol (FMA^+) at the protoplast surface is directly detected by the microelectrode tip scanned close to the sample surface in seawater containing ferrocenemethanol (FMA) and hydrogen peroxide an electron mediator and an enzyme-substrate, respectively.
The macrophages consume oxygen and produce ROS which become hydrogen peroxide by a dismutation reaction. The amount of hydrogen peroxide can be measured by the current obtained when the microelectrode is brought to about 〜50μm from the macrophage surface.
Teaching Experience
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化学反応速度論
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生物電気化学特論
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エネルギーデバイス工学